A quantitative study on free cholesterol, cholesterol ester and total cholesterol content of human gingiva in different degrees of chronic marginal periodontitis (author's transl)

Abstract

No report of lipids analysis of human gingiva in different degrees of chronic marginal periodontitis has so far appeared except OTA et al. of our department earlier reported. It was suggested that the content of cholesterol ester in human gingiva with periodontal disease revealed a tendency decrease.In the present study, the author investigated the measurement of content of free cholesterol, cholesterol ester and total cholesterol in the gingiva, that occured in association with the progress of chronic marginal periodontitis. At the same time, a qualitative analysis was also made to identify main fatty acid composition of cholesterol ester.A study material consisted of 19 males and 11 females, ranging from 18 to 50 in age, who were diagnosed as periodontitis in the department of periodontology, Nihon University Dental Hospital, and were divided into three clinical groups of P1, P2 and P3 by OKADA's classification. As controls, P0 group who were diagnosed as healthy gingiva consisted of 6 males and 4 females, ranging from 20 to 40 in age.The obtained gingival specimen was extracted for total lipids by FOLCH et al's method. An aliquot of total lipids was spotted on a Silicagel G glass plate. After developing, free cholesterol and cholesterol ester fractions were identified. Free cholesterol and cholesterol ester in appropriate areas of the individual fractions were then scraped off and re-extracted. Gas liquid chromatography was carried out for qualitative and quantitative analysis.Results were obtained as follows:1. The study on content of free cholesterol and total cholesterol in different degrees of chronic marginal periodontitis, there was no statistical significance among each clinical group.2. But the content of cholesterol ester revealed a tendency of decrease according to the severity of the disease, and it showed significantly different to compare P1 group with P2, P3 group.3. The main fatty acid composition of cholesterol ester was C14:0, C16:0, C16:1, C18:1 and C18:2. However, there was no change in different degrees of chronic marginal periodontitis.