A QUANTITATIVE HPLC ANALYSIS OF PHYTOCONSTITUENTS AND ASSESSMENT OF ANTIOXIDANT PROPERTIES OF THE RHIZOME OF Curcuma angustifolia ROXB

Abstract

The study undertaken aimed to rigorously standardize the primary phytochemical categories within the rhizome of Curcuma angustifolia, commonly referred to as Indian Arrowroot, by employing reverse-phase High-Performance Liquid Chromatography (HPLC). This research also ventured into assessing the antioxidant potential of rhizomederived extracts from Curcuma angustifolia. In this context, the ethanol extract of the Curcuma angustifolia Roxb. rhizome was meticulously analyzed for its content of vital phytochemicals such as flavonoids (0.013±0.78mg/ml), alkaloids (0.11±0.45mg/ml), and phenolics (4.08±0.08 mg/ml). The precision and accuracy of HPLC facilitated the identification and quantification of these significant compounds. Further, results from this study indicated that the extracts, with a particular emphasis on the ethanol extract, demonstrated notable antioxidant activities with IC50 values of 235.53±0.25µg/ml (DPPH Assay), 61.90±0.34µg/ml (Hydroxy Radical Assay), and 420.53±0.33µg/ml (Nitric Oxide Assay). This is primarily attributed to their capacity to scavenge free radicals effectively, highlighting the considerable potential of Curcuma angustifolia Roxb. for ethnomedicinal use and its prospective pharmacological applications. The findings lay the groundwork for further exploration into the traditional medicinal applications of Curcuma angustifolia Roxb. and its potential integration into pharmaceutical developments.