A Quantitative Estimation of Phytochemicals, Anti-Diabetic and Anti-Oxidant Activities of Crude Extracts of Sphagneticola trilobata (L.) and Adathoda vasica Linn

Abstract

Due to therapeutic efficacy, <i>Sphagneticola trilobata</i> (roots), and <i>Adathoda vasica</i> (whole plant) were selected to explore anti-diabetic and anti-oxidant activities of their crude plant extracts. The plant samples were extracted with low to high polar solvents viz. n-hexane, ethyl acetate, methanol and water. Further crude extracts were screened for the presence of phytochemicals such as alkaloids, flavonoids, phenols etc. The quantitative estimation of phenols, flavonoids, alkaloids was performed by spectrophotometric approach. The evaluation of anti-oxidant activity of <i>Sphagneticola trilobata</i> was determined by DPPH method and ABTS method was applied to access the antioxidant activity of Adathoda vasica. The antidiabetic activity of crude extracts of <i>Sphagneticola trilobata</i> and <i>Adathoda vasica</i> was determined along with <i>Brassica oleraceae</i> by applying in vitro α-amylase inhibition method. The methanolic extract of <i>Sphagneticola trilobata</i> was significantly showed DPPH IC50 inhibition at 20 μg/mL, and in vitro α-amylase inhibition IC50 at 50 μg/mL. In <i>Adathoda vasica </i>methanol extract showed ABTS inhibition IC50 at 30 μg/mL, and in vitro α-amylase inhibition of IC50 at 25 μg/mL. It is concluded that methanolic extract of <i>Sphagneticola trilobata</i> can be potential source of anti-oxidant and methanolic extract of <i>Adathoda vasica</i> proven as good anti-diabetic agent.<div><br></div>