Abstract
By the use of interference microscopy the rate of deposition of calcium phosphate, resulting from enzyme activity, can be measured quantitatively. This provides a quantitative measure of enzyme activity at individual cellular sites. By this method, studies have been made of the effect of variation in substrate concentration, pH, temperature and calcium-ion concentration on the enzymatic activity of the brush border regions of rat-kidney tubules and of duodenal epithelium. Some observations of cell nuclei have also been made. Under standard conditions of incubation (Danielli 1953) at least 97⋅5% of the phosphate ions liberated in a kidney section are precipitated, and once precipitated the calcium phosphate does not redissolve. Under standard conditions of incubation no loss of enzyme activity from brush borders of kidney and duodenum could be detected.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Proceedings of the Royal Society of London. Series B - Biological Sciences
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
