Abstract
Fast cyclic voltammetry has been used to study the relative importance of neuronal dopamine (DA) uptake and D 2 autoreceptor stimulation on the control of the extracellular concentration of electrically evoked DA in rat brain slices containing aCPu and NAc. The degree of potentiation of DA overflow by benztropine, cocaine and nomifensine was always greater in the aCPu than in the NAc. Although EC 50 values for the uptake blockers in the NAc were lower than in the aCPu, they were only significantly lower for 1 p with benztropine. One μM (-)-sulpiride, significantly potentiated DA overflow evoked by 20 p/20 Hz in both regions. In the presence of either 1 μM cocaine or 1 μM nomifensine, sulpiride further potentiated DA overflow in the NAc but not in the aCPu. The results indicate that in brain slices containing the aCPu, neuronal uptake is the main mechanism of controlling extracellular concentrations of DA due to electrical stimulation. Whilst in the NAc both uptake and D 2 autoreceptor activation are important control mechanisms. The rate of removal of DA from the extracellular medium was not significantly different in the two regions but was decreased in the presence of (-)-sulpiride. This indicates that D 2 receptors have a modulating effect on the DA neuronal uptake mechanism.
Published Version
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