Abstract

The quantitation of the efficacy of antimicrobial agents against Acanthamoeba requires the use of accurate and reliable quantitative methods. In the literature, data obtained by different methods conflict. Previous studies assessed efficacy against Acanthamoeba with several different methods: minimal inhibition of mobility after 6 hours of exposure [1]; simple presence or absence of motile trophozoites after 4 hours of contact [2]; number of cells per milliliter during 140 hours of growth in culture (method of cell count not given) [3]; hemocytometer counts of cells after 4, 24, and 72 hours of chemical contact [4]; and filtration of challenged solutions followed by culture for motile trophozoites after 24 hours of chemical contact [5]. Quantitative rates for the killing of Acanthamoeba cells are difficult to calculate from these methods because the number of viable cells

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