Abstract
Introduction. This study aimed to survey a total of five common human and veterinary antibiotics based on SPE-LC-MS-MS technology in a water treatment plant at central plateau of Iran. Also two sampling techniques, passive and grab samplings, were compared in the detection of selected antibiotics. Materials and Methods. In January to March 2012, grab and passive samples were taken from the influent and effluent of a water treatment plant. The samples were prepared using solid-phase extraction (SPE), and extracts were analyzed by liquid chromatography tandem mass spectrometry (LC-MS-MS). Results. The results showed that enrofloxacin, oxytetracycline, and tylosin were not detected in none of the samples. However, ampicillin was detected in the grab and passive samples taken from the influent (source water) of the plant, and ciprofloxacin was detected in passive samples taken from the influent and effluent (finished water) of the plant. Conclusion. The results imply that passive sampling is a better approach than grab sampling for the investigation of antibiotics in aquatic environments. The presence of ampicillin and ciprofloxacin in source water and finished water of the water treatment plant may lead to potential emergence of resistant bacteria that should be considered in future studies.
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