A qualitative and quantitative evaluation of smear layer removal efficacy of three different chelating agents using scanning electron microscopy and inductively coupled plasma mass spectrometry

Abstract

ABSTRACT Aim: The main goal of the present experimental research was to analyze the smear layer removal efficacy of chelating agents and correlate with the amount of calcium released from the radicular dentin with the canal space. Materials and Methods: Forty single-rooted teeth were exposed to standardized root canal instrumentation technique with copious amount of irrigation with 2.5% sodium hypochlorite solution between each instrumentation, following which the specimens were randomly allocated into four groups: Group I: 17% ethylenediaminetetraacetic acid (EDTA), Group II 0.2% Chitosan, Group III Qmix™, and Group IV Control group with no final irrigation. After the irrigation protocol, the specimens were subjected to the scanning electron microscopy (SEM) evaluation to determine the smear layer removal efficacy and induced plasma mass spectrophotometric analysis was done to quantify the amount of calcium ions content in the irrigating solution. The data were statistically analyzed using Kruskal–Wallis and Dunnett’s tests for smear layer removal analysis. Quantitative data were interpreted by the analysis of variance and Tukey’s-Karmer test for the spectroscopic data analysis. Results: The result of the SEM analysis showed that there was no significant difference among the smear layer removal efficacy between the experimental groups with P > 0.05. The quantitative spectrophotometric data showed that the highest calcium ions release was from the 17% EDTA group followed by 0.2% chitosan and lesser from the Qmix™ group. Conclusion: Within the limitation of this in vitro experimental research, all the three experimental groups showed a better smear layer removal efficacy. EDTA showed the greater amount of mineral release compared to Qmix™ and chitosan group.