Abstract

1. In corroboration of F. R. Lillie, it was found that the egg-secretions of Arbacia punclulata exert a chemotactic effect on sperm, and activate, agglutinate, and paralyze them.2. The egg secretions of Asterias forbesii behave in a similar manner toward Asterias sperm.3. Arbacia secretion activates, agglutinates, and paralyzes Asterias sperm, and Asterias secretion has the same effects on Arbacia sperm.4. Paralyzed sperm may be reactivated but not reagglutinated.5. The egg secretions test negatively for reducing substances and do not give the usual protein tests although they were found to be faintly positive to the acid tests and the xanthoproteic. This may be due to traces of the egg jelly.6. Agglutination may be gotten with dry egg powder.7. The agglutination reaction very possibly depends on a surface effect.8. More soluble substances escape from the egg of Arbacia in hypertonic sea-water and in sea-water infected with sperm, than from unfertilized eggs in normal sterile sea-water in the same length ...

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