A QCM-D-based biosensor for E. coli O157:H7 highlighting the relevance of the dissipation slope as a transduction signal

Abstract

A biosensor for detection of viable Escherichia coli (E. coli) O157:H7 is developed using a quartz crystal microbalance with dissipation monitoring (QCM-D). The detection platform is based on the immobilization of affinity purified polyclonal antibodies onto gold-coated QCM-D quartz crystals via a cysteamine self-assembled monolayer. QCM-D measurements conducted over a broad range of bacterial cell concentrations show that the optimal biosensor response is the initial slope of the dissipation shift as a function of elapsed time (Dslope). A highly log–log linear response in the initial Dslope is obtained for detection of E. coli O157:H7 over a wide range of cell concentrations from 3×105 to 1×109cells/mL. The prepared biosensor also exhibits a log–log linear working range from 107 to 109cells/mL for E. coli K12 D21, a non-pathogenic model organism and further shows satisfactory selectivity using Bacillus subtilis. To our knowledge, this is the first study demonstrating the use of the initial Dslope as a sensor response when using QCM-D technology.