A Pyrene-Tetrazole Fused Fluorescent Probe for Effective Real Time Detection Towards Aluminium Ion.

Abstract

We constructed a novel-binding site for metal ion detection using a fused tetrazole ring conjugated with aminopyrene (R). The designed structure of the molecule was successfully synthesized and determined the probe's selectivity by testing various metal ions and found that the probe effectively detects Al3+ ion visually. Checked the sensing ability of the probe with different approaches (fluorimetric and colorimetric), and the effectiveness is double confirmed. The added Al3+ ion to R procured a rapid change in color from yellowish orange to colorless. Under the UV lamp, a turn-on blue fluorescence was observed after adding aluminium ion, whereas the probe was non-fluorescent before detecting aluminium ion. The probable interface of the probe with aluminium ion has also been expected from HRMS spectral analysis results. The probe's utility in real-time monitoring of Al3+ ion in water is confirmed by a simple test kit prepared using filter paper. The kit showed a possible naked-eye detection with a notable color change, and when checked, the aluminium ion detected test kit under a UV lamp showed blue fluorescence.