A putative soybean GmsSOS1 confers enhanced salt tolerance to transgenic Arabidopsis sos1-1 mutant.

Abstract

The cDNA of GmsSOS1, a putative plasma membrane Na(+)/H(+) antiporter gene isolated from Glycine max, Glycine soja, and their hybrid, was constructed into plant expression vector pCAMBIA 1300 and then transformed with Agrobacterium tumefaciens under the control of CaMV 35S promoter to Arabidopsis thaliana wild-type (WT) and mutant (atsos1-1) plants. By hygromycin resistance detection and PCR analysis, transgenic plants (WT35S:GmsSOS1 and atsos1-1 35S:GmsSOS1) were obtained. Seed germination, seedling growth, and Na(+) contents in roots and shoots were analytically compared among WT, atsos1-1 mutant, and their transgenic lines under salt stress. The results showed that when GmsSOS1 was integrated into the genome of A. thaliana, the inhibitions of salt stress on seed germination and seedling growth were all significantly improved, and enhanced salt tolerance was displayed, which may be attributed to the decrease of Na(+) absorption in roots and transportation in shoots of the transgenic lines, especially for that of atsos1-1 mutant.