Abstract
BackgroundHigh salinity adversely affects crop production. Pyruvic acid is the precursor of abscisic acid (ABA) and other chemicals that are synthesized in chloroplast, some of which are involved in the response to salt. The transportation of pyruvic acid into chloroplast is mediated by pyruvate transporters. However, whether pyruvate transporters are involved in salt response has not been studied so far. Here, we answered this issue by assessing the function of a wheat pyruvate transporter in salt response.ResultsA pyruvate transporter TaBASS2 was isolated from salt-tolerant wheat cultivar Shanrong 3. The expression of TaBASS2 was induced by NaCl stress as well as H2O2 and ABA treatments. Constitutive expression of TaBASS2 in Arabidopsis bass2-1 mutant complemented the mevastatin-sensitive phenotype that reflects the deficiency of transporting pyruvic acid into chloroplast. Overexpression of TaBASS2 enhanced salinity tolerance and reactive oxygen species scavenging in wheat. Arabidopsis constitutively expressing TaBASS2 also exhibited enhanced tolerance to salinity and oxidative stress. In Arabidopsis, TaBASS2 repressed the expression of ABA INSENSITIVE 4 (ABI4), a node linking ABA signaling and plastid retrograde signaling pathways. However, the enhanced salinity tolerance of TaBASS2 overexpression Arabidopsis was abolished when ABI4 expression was restored to the level of wild-type through overexpressing ABI4.ConclusionsOur work demonstrates that TaBASS2 enhances salinity tolerance of plants via modulating ABI4 expression. This indicates that pyruvate transporters indeed participate in the interaction of plants with environmental stimuli.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-016-0795-3) contains supplementary material, which is available to authorized users.
Highlights
IntroductionWhether pyruvate transporters are involved in salt response has not been studied so far
TaBASS2 is induced by NaCl treatment To determine the expression pattern of TaBASS2 under salinity stress, three-leaf-stage Shanrong No 3 (SR3) seedlings were treated with 200 mM NaCl solution and monitored TaBASS2 expression in roots
These results demonstrated that TaBASS2 expression responded to high level of salinity as well as other stress signals
Summary
Whether pyruvate transporters are involved in salt response has not been studied so far We answered this issue by assessing the function of a wheat pyruvate transporter in salt response. In the past few decades, extensive studies on salinity stress in plants, especially in the model plant Arabidopsis thaliana, have uncovered a number of genes involved in plant salt tolerance [2, 3]. Both abscisic acid (ABA)dependent and -independent signaling pathways play important roles during this process [4, 5]. Three loss-of-function mutations in ABI4 conferred increased tolerance in both seedling and
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