STAR protocols | VOL. 3
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A protocol for CRISPR-mediated activation and repressionof human endogenous retroviruses in human pluripotent stem cells.

Publication Date Jun 17, 2022

Abstract

Human endogenous retroviruses (HERVs) comprise many regulatory elements and can regulate host gene activity at different expression levels via multiple mechanisms. Here, we introduce a step-by-step protocol to activate or repress transcription of HERV-K(HML-2) elements using the CRISPRa and CRISPRi technologies in human embryonic stem cells. This protocol can help deciphering the functional role of HERV-K(HML-2) elements in critical biological processes. The protocol may easily be adapted to other cell lines and HERV groups with relatively low sequence heterogeneity. For complete details on the use and execution of this protocol, please refer to Padmanabhan Nair etal. (2021).

Concepts

Human Endogenous Retroviruses Human Endogenous Retroviruses Groups HERV-K Elements Human Pluripotent Stem Cells Human Embryonic Stem Cells Regulatory Elements Activation In Stem Cells Multiple Mechanisms Stem Cells Elements In Processes

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No potential conflict of interest was reported by the authors. The conception and design of the study, acquisition of data, analysis and interpretatio...

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