Abstract
We established a quantitative reporter gene protocol, the P/Rluc assay system, allowing the sequential measurement of Photinus and Renilla luciferase activities from the same extract. Other than comparable commercial reporter assay systems and their noncommercial counterparts, the P/Rluc assay system was formulated under the aspect of full compatibility with standard methods for protein assays. This feature greatly expands the range of applications for assay systems quantifying the expression of multiple luciferase reporters.
Highlights
Pectenotoxins (PTXs) are a group of marine biotoxins whose structure is based on polyether lactones produced by a restricted variety of phytoplanktonic species [1,2,3]
We compared the metabolism of PTX-2 by human and rat liver S9 fractions, and we investigated the capability of PTX-2: (i) to modulate the gene expression of a panel of Phase I
9, 212 could be 2017, found (Figure 2a,b). Since these data strongly suggest a major role for Phase I enzymes in the metabolism of PTX-2, we. Since these data strongly suggest a major role for Phase I enzymes in the metabolism of PTX-2, investigated whether PTX-2 may modulate xenobiotic-metabolizing enzyme (XME) gene expressions we investigated whether PTX-2 may modulate xenobiotic-metabolizing enzyme (XME) gene in liver cells such as the metabolic competent expressions in liver cells such as the metabolic HepaRG
Summary
Pectenotoxins (PTXs) are a group of marine biotoxins whose structure is based on polyether lactones produced by a restricted variety of phytoplanktonic species [1,2,3]. Among this group, pectenotoxin 2 (PTX-2, see Figure 1) is the best documented compound. Toxins 2017, 9, 212 hepatic damage after intraperitoneal injection [7]. We previously showed that PTX-2 was cytotoxic in the human metabolic competent hepatoma cell line HepaRG, inducing apoptosis and DNA damage [11]. We showed that PTX-2 failed to induce PXR translocation in HepG2 cells [11]
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