A Proteomic View of the Cross-Talk Between Early Intestinal Microbiota and Poultry Immune System.

D R Rodrigues,K M Chasser,K M Wilson,W G Bottje,M Trombetta,A F Duff,L Bielke,W N Briggs

doi:10.3389/fphys.2020.00020

D R Rodrigues, K M Chasser + Show 6 more

Open Access

https://doi.org/10.3389/fphys.2020.00020

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Abstract

Proteomics has been used to investigate cross-talk between the intestinal microbiome and host biological processes. In this study, an in ovo technique and a proteomics approach was used to address how early bacterial colonization in the gastrointestinal tract (GIT) could modulate inflammatory and immune responses in young broilers. Embryos at 18 embryogenic days were inoculated with saline (S), 102 CFU of Citrobacter freundii (CF), Citrobacter species (C2), or lactic acid bacteria mixture (L) into the amnion. At 10 days posthatch, ileum samples from 12 birds per treatment were selected for tandem mass spectrometry analysis. Our further findings indicated that treatment-specific influences on early GIT microbiota resulted in different immune responses in mature broilers. Predicted functional analyses revealed activation of inflammation pathways in broilers treated in ovo with L and CF. Exposure to L enhanced functional annotation related to activation, trafficking of immune cells, and skeletal growth based-network, while CF inhibited biological functions associated with immune cell migration and inflammatory response. These results highlighted that proper immune function was dependent on specific GIT microbiota profiles, in which early-life exposure to L-based probiotic may have modulated the immune functions, whereas neonatal colonization of Enterobacteriaceae strains may have led to immune dysregulation associated with chronic inflammation.

Highlights

Pioneer colonization of intestinal microbiota has a major effect on driving the maturation and composition course of microbial communities over time (Juricova et al, 2013; Rodrigues et al, 2019; Wilson et al, 2019)
We focused on intestinal inflammatory and immune-related proteins, screened the biological functions predicted by Ingenuity Pathway Analysis (IPA), and linked inflammation biomarkers to intestinal microbial signatures established in the ileal microbiome of 10-day-old broiler chickens
We identified 617 proteins in L, 613 proteins in Citrobacter freundii (CF), and 625 proteins in C2 (Supplementary Tables S2–S4, respectively)

Summary

Introduction

Pioneer colonization of intestinal microbiota has a major effect on driving the maturation and composition course of microbial communities over time (Juricova et al, 2013; Rodrigues et al, 2019; Wilson et al, 2019). The cross-talk between microbiota composition and immune cells has been highly associated with the establishment of immune competence (Crhanova et al, 2011; Chung et al, 2012; Schokker et al, 2017; Duan, 2018). The lack of intestinal microbiota in these mice caused extensive deficits in the development of the gut-associated lymphoid tissues, abnormal production of immune cells, and other immunological deficiencies (Round and Mazmanian, 2009). In this context, a recent study with broilers has. The specific microbial populations involved in immune-modulatory functions are beginning to be deciphered with the advancement of metagenomic analyses

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