Abstract
Diabetes mellitus is a widespread metabolic disorder, and long-term hyperglycemia in diabetics leads to diabetic keratopathy. In the present study, we used a shotgun liquid chromatography/mass spectrometry-based global proteomic approach using the cornea of streptozotocin-induced diabetic (STZ) rats to examine the mechanisms of delayed corneal wound healing in diabetic keratopathy. Applying a label-free quantitation method based on spectral counting, we identified 188 proteins that showed expression changes of >2.0-fold in the cornea of STZ rats. In particular, the level of lumican expression in the cornea of STZ rats was higher than that of the normal rats. In the cornea of the normal rat, the expression level of lumican was elevated during the wound healing process, and it returned to the same expression level as before cornea injury after the wound was healed completely. On the other hand, a high expression level of lumican in the cornea of STZ rats was still maintained even after the wound was healed completely. In addition, adhesion deficiency in corneal basal cells and Bowman’s membrane was observed in the STZ rat. Thus, abnormally overexpressed lumican may lead to adhesion deficiency in the cornea of STZ rats.
Highlights
Diabetes mellitus (DM) is a widespread metabolic disorder
DM is characterized by insulin resistance and impaired insulin secretion [2], and the resulting long-term hyperglycemia in diabetics leads to many ophthalmic complications, including retinopathy, cataracts, uveitis, and keratopathy [3]
We examined the changes in expression of lumican protein in the cornea of normal and streptozotocin-induced diabetic (STZ) rats during the wound healing process
Summary
Diabetes mellitus (DM) is a widespread metabolic disorder. The world prevalence of DM among adults (aged 20 to 79 years old) in 2010 was 6.4% (285 million adults), and will increase to 7.7% (439 million adults) by 2030 [1]. Diabetic keratopathy shows relatively few symptoms compared with other ophthalmic complications of DM; once the cornea is damaged, delayed corneal wound healing is often observed [4]. Changes in the expression of these proteins are key factors in the process of corneal wound healing in Iinnth. C36h35anges in the expression of these proteins are key factors in the proce2ssof o14f corneal wound healing in diabetic keratopathy due to the modulation of cell adhesion or migration diniatbheeticcokrneeraat.oIpnaatdhydidtiuoen,tothteheexmtroadceulllautliaornmofatcreilxl (aEdChMes)ioinn othremciogrrnaetiao(ncoinnstihseticnogrnofeag.lyIncoapdrdoitteiionns, tahnedexptrroatceeollgullyacramn)atirsixe(sEseCnMti)alinfothretchoernmeaai(nctoennsaisntcinegooff cgolyrncoeaplrottreainnsspaanrdenpcryo,teaongdlycchana)nigseesssinentthiael fporrottheeogmlyacinatnencoanncteenotfocforthneeaclotrrnaneaspmaraeyncrey,saunltdinchcaonrgneesailnotphaecpitryot[e2o1g–l2y3c]a. AA VVeennnn mmaapp ooff pprrootteeiinnss iiddeennttiiffiieedd ffrroomm tthhee ccoorrnneeaa ooff nnoorrmmaall aanndd ssttrreeppttoozzoottoocciinn ((SSTTZZ))--iinndduucceedd ddiiaabbeettiicc rraattss. EAipnodsiictiavteedRsrcevdauluceedindexicpatreedssiinocnreiansetdheexcporersnseioanoifndthiaebetic rat (licgohrnt egaroafydaiarebaet)i.c Irnat,aadndditaionneg, atthiveenvoarlumeailnidzeicdatsepdercetdruacleadbeuxnpdreasnsicoen fianctthoerc(oNrnSeAa Fof) dviaalbueeticforarteach protei(nligihdtengtriafiyedariena)t.hIenNaodrdmitiaolna, nthdeSTnoZrmgraoliuzpeds wspaesctarlasloacbaulncudlaantceed.faPcrtoorte(iNnsSAwFi)thvaalhueighforpoesaicthive or protein identified in the Normal and STZ groups was calculated. 15 p3roteins claQs6siPfi9eTd8 as “extracellular Tmuabturilxin”—bewtah-4icBhchpalainys important roles i−n 2c.0o6rn2ea wound hofeaelni456nhga-nicneGd OexcPPPpe066rle511lus197sl985ai701orncoomf lpuomnHiecenattnetreionrgmetAhnseeD(oTcEPuoal-sobrrninlnbeeguoa1acs)tloyei.oflaAanrStmiTrfoiaZobncnotsfongainructct2choeleerlo1u4p5mropitcreaointneiiKns sa, owneefoofcu−−−ths211ee...d055k600oe255yn the effect factors in the w7ound healPin62g2p7r1ocess of the corne4a0.S ribosomal protein S18
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
