A protein which masks galactose receptor mediated phage susceptibility in Lactococcus lactis subsp. lactis MPL56

Abstract

A 28.5‐kb plasmid, isolated from Lactococcus lactis subsp. lactis MPL56, causes complete inhibition of four lactococcal phages. Cell wall characteristics of wild‐type strain MPL56 were compared with its 28.5 kb plasmid‐cured, phage‐sensitive derivative MPL56‐22. After proteolytic enzyme treatments, adsorption of phages occurred at high levels, an example is 94.6–98.5% in MPL56 cells. Analysis of cell wall extracts of MPL56‐22 by sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE) indicated that the only difference between strains was the 55.4 kDa band in protein patterns of MPL56. Adsorption of the four phages was completely inhibited when MPL56‐22 cells were subjected to SDS, Triton‐X‐100, HCl and NaOH treatments. Lectins that were specific for glucose/mannose and N‐acetylglucosamine did not prevent adsorption of phages in cell wall extracts of MPL 56‐22. However a lectin specific for galactose (MCA; Momordica charantia) completely inhibited adsorption of these phages in cell wall extracts of MPL56‐22. HPLC patterns of cell wall carbohydrates of MPL56‐22 and its HCl treated preparations showed that the most prevalent difference was the galactose on untreated MPL56‐22 cell wall chromatograms.