Abstract
Summary Rat giant trophoblast cells contain a factor which stimulates DNA polymerase α derived from trophoblast cells as well as from calf thymus. The factor, which has been partially purified on a glycerol gradient, has an approximate M r of 85,000 and is non-dialyzable, heat-labile, sensitive to trypsin and insensitive to N-ethylmaleimide. With activated DNA as the preferred template/primer, the factor stimulates both the initial rate and the extent of DNA synthesis as a linear function of the concentration of the factor. The stimulation is abolished by aphidicol in but is unaffected by 2′, 3′ dideoxythymidine triphosphate.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
