Abstract
2M NaCl-insoluble fraction of rat ventral prostate chromatin (residual proteins) contain proteins able to interact specifically with androgen-receptor complex and is, therefore, a part of the acceptor complex. Among residual proteins, a 97 KDa protein has been found which binds significantly to a genomic fiagment containing an androgen-regulated gene coding for a 22 KDa protein. The biological significance of this binding in androgen action need to be further studied. A mini-plasmid clone containing 22 KDa protein coding sequence was cloned into Charon 4A genomic library from which a 5.7 Kb genomic fragment was isolated, identified by hybridization with a 5′ and a 3′ cDNA probes, and shown to contain the 5′ flanking sequence. Restriction enzyme treatment of this fragment yielded a 4.7 Kb restriction fragment representing the 5′ upstream region and a 1.0 Kb containing part of the coding sequence. Deletion studies indicated that the 97 KDa protein bound only to a subclone of about 300 bp segment. Furthermore, gel shifting experiment supported its DNA-protein binding.
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