Abstract
Objectives: Removal of a single blastomere for preimplantation genetic diagnosis (PGD) from day-3 embryo is a well established method. However, it is unknown whether the method of choice for zona pellucida (ZP) drilling for the biopsy procedure will influence further embryonic development. The most commonly applied technique for perforation of the ZP is zona drilling using acidified Tyrode’s solution (AT), while only a few centers employ laser-assisted hatching (LAH). Because of the lack of studies comparing the two methods, many centers are reluctant to switch to the simpler and faster laser drilling. Thus, in the present study we assessed two zona drilling methods in terms of blastocyst development rates using sister embryos.
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