A proposed metabolic pathway mechanism along with Lactobacillus acidophilus growth modeling in a novel semi-artificial biohybrid system based on CdS-NPs

Abstract

A metabolic pathway mechanism in a newly developed semi-artificial biohybrid system (SABS) constructed from Lactobacillus acidophilus and cadmium sulfide nanoparticles (CdS-NPs) was proposed. Also, the bacterium growth in the biohybrid system was modeled via historical data design (HDD). The findings showed that the bacterial biomass production in the biosystem occurs in several steps, including Light harvesting, Electron generation, Transduction, Glycolysis pathway, Electro-fermentation, Substrate for biomass accumulation, Gluconeogenesis pathway and anabolism, and Biomass production. In general, electron transfer (ET) increases the nicotinamide adenine dinucleotide (NADH/NAD+) ratio, accumulates pyruvate, generates more adenosine triphosphate (ATP), and incites the bacterium toward more growth. The modeling with HDD indicated that the correlation between time and absorbance in the biohybrid system corresponded with a cubic model with R2 > 0.99 and the optimum point of 1.93 at the time of 64.14 h.