A proof-of-concept study on the versatility of liquid chromatography coupled to high-resolution mass spectrometry to screen for various contaminants and highlight markers of floral and geographical origin for different honeys

Abstract

The high-resolution mass spectrometry is a powerful analytical tool for improving food safety and authenticity, but still underused in official control laboratories. The present work is a proof-of-concept study overviewing how liquid-chromatography coupled to high-resolution mass spectrometry could be used simultaneously for large-scale screening of contaminants and differentiation of honey samples. Within this study, the samples were extracted using all-in-one QuEChERS-based protocol that allowed for analysis of various anthropogenic contaminants and endogenous compounds. First, targeted-analysis of 52 honey samples led to unequivocal identification of 23 chemicals, including neonicotinoids, triazole fungicides and synergist. Then, suspect-screening using MSDial software allowed for tentative identification of 30 chemicals including plasticizers, flame-retardants and additives. Suspect-screening also made it possible to highlight tentative markers of chestnut honey (deoxyvasicinone, 2-quinolone, indoleacrylic acid and kynurenic acid) and citrus honey (caffeine, 2-oxindole and indole-3-carbinol). Lastly, non-targeted analysis enabled to separate honeys by their type, floral and geographical origins.