Abstract
Diabetes and hypertension are complex pathologies with increasing prevalence nowadays. Their interconnected pathways are frequently manifested in retinopathies. Severe retinal consequences and their tight connections as well as their possible treatments are particularly important to retinal research. In the present, work we induced diabetes with streptozotocin in spontaneously hypertensive rats and treated them either with PACAP or olaparib and alternatively with both agents. Morphological and immunohistochemical analyses were carried out to describe cell-specific changes during pathologies and after different treatments. Diabetes and hypertension caused massive structural and cellular changes especially when they were elicited together. Hypertension was crucial in the formation of ONL and OPL damage while diabetes caused significant differences in retinal thickness, OPL thickness and in the cell number of the GCL. In diabetes, double neuroprotective treatment ameliorated changes of calbindin-positive cells, rod bipolar cells and dopaminergic amacrine cells. Double treatment was curative in hypertensive diabetic rat retinas, especially in the case of rod bipolar and parvalbumin-positive cells compared to untreated or single-treated retinas. Our results highlighted the promising therapeutic benefits of olaparib and PACAP in these severe metabolic retinal disorders.
Highlights
Published: 9 December 2021Diabetes mellitus and hypertension are two common, chronic, multifactorial diseases with increasing prevalence and socioeconomic complications worldwide
Was injected into the vitreous body of the right eye with a Hamilton syringe three times under anesthesia, the same volume of saline was injected into the left eye, while olaparib was applied through their drinking water
The presence of retinal diseases or effect of different treatments resulted in an alteration in the appearance of retinal layers between groups listed below
Summary
Diabetes mellitus and hypertension are two common, chronic, multifactorial diseases with increasing prevalence and socioeconomic complications worldwide. They both tend to appear with increased frequency as the aging process advances and usually coexist in clinical practice, with elevated prevalence for hypertension in diabetic patients. Diabetic retinopathy (DR) is a major well-known cause for blindness with an increasing tendency in the working population [10,11].
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
