Abstract
An earlier report described separation of crustacean eyestalk extract by gel filtration on G-25 Sephadex into two groups of pigmentary-effector hormones. One contains distal retinal pigment light-adapting hormone (DRPH) and melanophore dispersing hormone (MDH) in Peak 1B and the other an erythrophore concentrating hormone (ECH) in Peak 4. This is a report of progress in further purification of such hormones. Peak 1B material also disperses the pigment in leucophores and erythrophores. Further chromatography of Peak 1B material on two ion-exchangers shows the separation of several different molecular versions of a particular pigmentary-effector hormone. In addition, each active peak is effective on all four pigmentary effectors, causing light-adaptation of distal retinal pigment and dispersing the pigments in melanophores, leucophores, and erythrophores. Homogeneity of these preparations has not yet been thoroughly examined, so that it is not yet possible to state whether each active peak contains one or several pigmentary effector hormones.
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