Abstract

Spectral reconstruction method based on narrow-band measurements has been demonstrated to achieve ultrafast spectroscopic imaging with high spatial and spectral resolution, in which multiple narrow-band images are collected by using several specific filters. Although commercially available filters can be employed in such method, filters with complex transmittance that are difficult to be fabricated typically show significant improvement in spectral reconstruction accuracy. In this study, a two dimensional programmable optical filter based on digital micromirror device (DMD) is proposed, in which its transmittance spectrum can be arbitrarily and quickly switched to realize complex transmittance. Furthermore, its flexible transmittance enables directly hardware-based spectral data post-processing, which can perform data acquisition and analysis simultaneously. Those have been evaluated by the diffuse reflectance spectra from normal and occluded skin flaps, as well as Raman spectra from live, apoptosis and necrosis leukemia cells. Our simulation results show that much higher spectral reconstruction accuracies can be achieved by the optimized filters with complex transmittance. Furthermore, the classification accuracy by using the proposed method is comparable to those achieved by conventional numerical methods. Therefore, based on the proposed programmable optical filter, fast spectroscopic imaging with high spatial and spectral resolution can be achieved for observing fast changing phenomena and even real-time target identification.

Highlights

  • Spectral imaging technique combines spectroscopy with conventional imaging, in which both spatial and spectral information can be collected simultaneously to form a three-dimensional spectral data cube [1]

  • Excellent classification accuracies were achieved, which are comparable to those of conventional numerical methods. Our contributions in this preliminary study can be summarized as: 1) a two-dimensional programmable optical filter with arbitrary transmittance is proposed, and its robustness with non-ideal incidence beams is theoretically investigated; 2) the spectral reconstruction accuracies for spectral reconstruction based spectroscopic imaging technique can be significantly improved by the complex transmittance of the proposed programmable optical filter; 3) hardware-based spectral data post-processing is theoretically investigated to substitute for the conventional numerical methods, the spectral data acquisition and VOLUME 7, 2019

  • The focal length was optimized to 35mm and the groove density was set to 800 lines/mm

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Summary

INTRODUCTION

Spectral imaging technique combines spectroscopy with conventional imaging, in which both spatial and spectral information can be collected simultaneously to form a three-dimensional spectral data cube [1]. Programmable optical filter with specific transmittance was explored to perform hardware-based spectral data post-processing, in which narrow-band measurements were directly treated as the final coefficients for target identification instead of numerical post-processing followed by spectral data acquisition. Our contributions in this preliminary study can be summarized as: 1) a two-dimensional programmable optical filter with arbitrary transmittance is proposed, and its robustness with non-ideal incidence beams is theoretically investigated; 2) the spectral reconstruction accuracies for spectral reconstruction based spectroscopic imaging technique can be significantly improved by the complex transmittance of the proposed programmable optical filter; 3) hardware-based spectral data post-processing is theoretically investigated to substitute for the conventional numerical methods, the spectral data acquisition and VOLUME 7, 2019.

MATERIALS AND METHODS
PROGRAMMABLE OPTICAL FILTER BASED FAST SPECTROSCOPIC IMAGING
PROGRAMMABLE OPTICAL FILTER BASED SPECTRAL DATA POST-PROCESSING
RESULTS AND DISCUSSIONS
CONCLUSSION
Full Text
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