Abstract
Recent studies have indicated that long non-coding RNAs (lncRNAs) may participate in the regulation of tumor cell proptosis. However, the connection between lncRNA expression and pyroptosis remains unclear in colon adenocarcinoma (COAD). This study aims to explore and establish a prognostic signature of COAD based on the pyroptosis-related lncRNAs. We identify 15 prognostic pyroptosis-related lncRNAs (ZNF667-AS1, OIP5-AS1, AL118506.1, AF117829.1, POC1B-AS1, CCDC18-AS1, THUMPD3-AS1, FLNB-AS1, SNHG11, HCG18, AL021707.2, UGDH-AS1, LINC00641, FGD5-AS1 and AC245452.1) from the TCGA-COAD dataset and use them to construct the risk model. After then, this pyroptosis-related lncRNA signature is validated in patients from the GSE17536 dataset. The COAD patients are divided into low-risk and high-risk groups by setting the median risk score as the cut-off point and represented differences in the immune microenvironment. Hence, we construct the immune risk model based on the infiltration levels of ssGSEA immune cells. Interestingly, the risk model and immune risk model are both independent prognostic risk factors. Therefore, a nomogram combined risk score, immune risk score with clinical information which is meaningful in univariate and multivariate Cox regression analysis is established to predict the overall survival (OS) of COAD patients. In general, the signature consisted of 15 pyroptosis-related lncRNAs and was proved to be associated with the immune landscape of COAD patients.
Highlights
Colorectal cancer (CRC) is one of the most frequent malignancies and the third leading cause of cancer-related death globally
After taking the 118 pyroptosis-related long non-coding RNAs (lncRNAs) into multivariate Cox analysis (p < 0.05) and difference analysis (p < 0.05), we identified that 19 of them were associated with prognosis
In the multivariate Cox analysis, we found that N stage, M stage, AJCC stage, risk score and immune risk score were significantly associated with prognosis, consistent with the result of univariate Cox analysis (Table 2)
Summary
Colorectal cancer (CRC) is one of the most frequent malignancies and the third leading cause of cancer-related death globally. Based on the pathologic classification, colon adenocarcinoma (COAD) is the most common subtype of colon cancer (Sui et al, 2020; Yu et al, 2020). The incidence of COAD has been rising among adolescents. The 5-years overall survival (OS) rate of COAD patients without distant metastasis has a good prognosis, but that of patients with metastases is less than 15% (Almatroudi, 2020; Gan et al, 2020). The AJCC TNM staging system as the prognostic indicator of COAD patients is continually updated, a significantly different prognosis still exists in patients with the same clinicopathologic characteristics Identifying specific and sensitive prognostic biomarkers is imperative to predict the outcomes of COAD patients
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