Abstract
Radiolabelled maltose and tetanus toxoid were each entrapped in multilamellar dehydration-rehydration vesicles (DRVs) composed of egg phosphatidylcholine (up to 66 μmol) and equimolar cholesterol. Solute-containing DRVs were microfluidized to smaller vesicles (to a mean diameter of around 100 nm, as measured by photon correlation spectroscopy) which retained 10–100% of the originally entrapped solute. Solute retention was found to be dependent on the number of microfluidization cycles, the medium in which microfluidization was carried out and on whether or not unentrapped solute was removed before processing. Under appropriate conditions, vesicles with mean diameters of less than 200 nm were produced, retaining about 35–78% of the originally entrapped solute. These would be suitable for in vivo use where small vesicle size and an augmented entrapped solute to liposomal lipid mass ratio are required.
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