Abstract
Here we present a one-pot procedure for the synthesis of a library of carbohydrate compounds belonging to four classes of glycosidase inhibitors: glycosides, aldonic acid lactams, aldonic acid lactones and dideoxy-iminoalditols. The complex mixture was separated into individual components that were screened for activity against α- and β-glucosidases. Among these were two 2-O-methyl glycosides that reduced the rate of the enzymatically-catalyzed hydrolysis of the p-nitrophenyl glucosides used in the assay. Relative inhibition indices normalized to the inhibition observed with deoxynojirimycin under similar conditions were determined. This methodology provides a straightforward, general and time-efficient way to synthesize, test and analyze an extensive number of glycosidase inhibitor candidates from several carbohydrate compound classes in one scheme. The direct reference to deoxynojirimycin gives an immediate indication of the concentration range in which a significant inhibitory activity can be obtained.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.