Abstract
ObjectiveCoronary artery disease (CAD) is a multi-factor disease. Complement component 3 (C3) plays an important role in the development of CAD. The present study investigated the association between DNA methylation status of C3 gene promoter and the risk of CAD.MethodsOne hundred CAD patients and 1 hundred age-and gender- matched controls were recruited in current study. Methylation levels in CpG island in C3 promoter were determined by the method of bisulfite amplicon sequencing.ResultsMethylation levels of four CpG sites in C3 promoter were measured. There were no significant difference in methylation level of each CpG site between CAD patients and controls. Average methylation rate was also calculated. No significant difference in average methylation rate was observed between CAD and control groups. Stratified analyses based on EH, DM and smoking status were carried out, no significant association between C3 promoter methylation levels and the susceptibility of CAD was observed. Furthermore, seven haplotypes were established and no significant difference in haplotypes was observed between CAD and control groups. However, our study showed that C3 DNA methylation levels were positively associated with LDL-C levels.ConclusionThe present study showed no association between methylation levels of C3 promoter and the risk of CAD. However, the methylation levels might be related to LDL-C levels.
Highlights
Coronary artery disease (CAD) remains the leading cause of morbidity and mortality worldwide
There were no significant difference in methylation level of each CpG site between CAD patients and controls
No significant difference in average methylation rate was observed between CAD and control groups (Fig. 2)
Summary
Coronary artery disease (CAD) remains the leading cause of morbidity and mortality worldwide. With the rapid aging of the population, increased calorie consumption and decreased physical activity, the incidence of cardiovascular disease in some developing countries will increase steadily in future [1]. The interaction among environmental, genetic and epigenetic factors affects the occurrence and development of CAD, which has been accepted widely [2]. Epigenetic regulation, changing the expression of genes without changing the DNA sequence, plays an important role in the occurrence of various diseases. The research on the association between epigenetics and cardiovascular disease was investigated more and more deeperly [3]. Candidate genes and epigenome-wide association studies (EWAS) showed that DNA methylation, the most common and important epigenetic modification pattern, was related to cardiovascular disease [4,5,6]
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