A Preliminary Study on the Relationship between Parasitaemia and Cytokine Expression of Peripheral Blood Cells in Trypanosoma vivax-Experimentally Infected Cattle.

Abstract

Simple SummaryInfections by Trypanosoma vivax in livestock have been reported with increasing frequency worldwide. Nevertheless, information regarding the immune response during the infection is scarce. Regarding that, cytokines play an important role as inflammation modulators, influencing the outcome of trypanosomosis. This study aimed to evaluate host cytokine production during T. vivax infection, in order to assess the increase or decrease of selected cytokines with the cattle’s ability to control the infection. While animals that showed an increase in IL-6 and IFNγ managed T. vivax parasitaemia satisfactorily, cattle that showed reduction of IL-1β, IL-2 and TNFα did not control the parasite multiplication. The presented results are preliminary and shed some light on the role of cytokines during T. vivax-infection.Trypanosoma vivax outbreaks have been reported with increasing frequency worldwide, causing significant economic losses in livestock. Though several studies have suggested that cytokine responses may influence infection caused by Trypanosoma sp., their exact role remains unclear and may vary according to the animal species and parasite strain. The present study aimed to evaluate cytokine expression of peripheral blood cells from three Girolando dairy cows experimentally infected with T. vivax. For this purpose, blood samples were collected prior to the inoculation on the day of inoculation (D0), the day after inoculation (D1), and then every seven days up to 119 days after infection (DAI). Each animal presented a unique pattern of cytokine expression. While a tendency of a Th1 cytokine response was observed during the patent phase (presence of circulating parasites), an increase of Th2 cytokine expression was found at the beginning of the sub-patent phase (low parasitaemia or aparasitaemic periods). In animals that presented a better control of parasitaemia, IL-6 and IFNγ increased during most of the trial period. On the other hand, the cow that presented reduction of IL-1β, IL-2, and TNFα during the entire period did not control parasitaemia properly. A balance between the Th1 and Th2 profile is beneficial for parasite control and animal health. The results found in the present study are a first step towards elucidating the dynamics of cattle’s inflammatory response against T. vivax, requiring future studies focusing on the role of key cytokines on the controlling of parasitaemia in different stages of bovine trypanosomosis.