Abstract
Aim. The purpose of this study was to determine the in vitro response of cells critical to the wound healing process in culture media supplemented with a lyophilized preparation rich in growth factors (PRGF) and Manuka honey. Materials and Methods. This study utilized cell culture media supplemented with PRGF, as well as whole Manuka honey and the medical-grade Medihoney (MH), a Manuka honey product. The response of human fibroblasts (hDF), macrophages, and endothelial cells (hPMEC) was evaluated, with respect to cell proliferation, chemotaxis, collagen matrix production, and angiogenic potential, when subjected to culture with media containing PRGF, MH, Manuka honey, and a combination of PRGF and MH. Results. All three cell types demonstrated increases in cellular activity in the presence of PRGF, with further increases in activity seen in the presence of PRGF+MH. hDFs proved to be the most positively responsive cells, as they experienced enhanced proliferation, collagen matrix production, and migration into an in vitro wound healing model with the PRGF+MH-supplemented media. Conclusion. This preliminary in vitro study is the first to evaluate the combination of PRGF and Manuka honey, two products with the potential to increase regeneration individually, as a combined product to enhance dermal regeneration.
Highlights
The dermal healing response is a multistep process which may result in a number of different outcomes: complete healing, scarred healing, or a chronic nonhealing wound [1, 2].In nonhealing wounds, such as pressure and diabetic ulcers, the carefully coordinated wound healing process has been altered
This aberrant set of regulatory signals has far-reaching effects on all the cells involved in dermal healing and results in increased proteolytic activity and improper extracellular matrix (ECM) deposition
Statistical analysis revealed there to be no significant differences in activated TGF-β regardless of whether Platelet-rich plasma (PRP) was activated through acid treatment or mixing with MH
Summary
The dermal healing response is a multistep process (inflammation, granulation tissue proliferation, epithelialization, and remodeling of the wound site) which may result in a number of different outcomes: complete healing, scarred healing, or a chronic nonhealing wound [1, 2].In nonhealing wounds, such as pressure and diabetic ulcers, the carefully coordinated wound healing process has been altered. The dermal healing response is a multistep process (inflammation, granulation tissue proliferation, epithelialization, and remodeling of the wound site) which may result in a number of different outcomes: complete healing, scarred healing, or a chronic nonhealing wound [1, 2]. While inflammation normally resolves within 1-2 days as neutrophil number decreases, the prolonged presence of these cells contributes to a disordered network of regulatory cytokines This aberrant set of regulatory signals has far-reaching effects on all the cells involved in dermal healing (macrophages, fibroblasts, etc.) and results in increased proteolytic activity and improper extracellular matrix (ECM) deposition. In order for these wounds to heal, the self-propagating loop of chronic inflammation must be disrupted. To date, there has been no single treatment that has proven to be optimal at stimulating the resolution of chronic wounds, and the future
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