Abstract
Objective To observe the effect of inhibition of the differentiation of colon cancer cells initiated by the interference of anti-Foxp3dsFv-IL-6 fusion protein on the imbalance of Treg/Th17. Methods Clean female BALB/c mice were divided into 3 groups. Each group was subcutaneously inoculated with colon cancer cell line CT26. After 1 week, the mice in group A were injected with anti-Foxp3dsFv-IL-6 fusion protein, and the mice in group B were injected with PBS, and the mice in group C were not treated. In vivo Treg/Th17 state were detected in each group of mice in the following three time points: in the initial stage when the colon cancer cell line was injected, when anti-Foxp3dsFv- IL-6 fusion protein or PBS was injected and 1 week after the injection. At the same time, the survival of each group of mice were observed. Results In group A, after the mice were injected with anti- Foxp3dsFv-IL-6 fusion protein, the proportion of Treg decreased and the proportion of Th17 cells increased, and the ratio of Treg/Th17 decreased. While in group B and group C, the ratio of Treg/Th17 increased. The survival time of group A, group B and group C were (24.9±2.7) d, (16.4±1.8) d and (16.8±1.5) d, respectively. Analysis of One-Way ANOVA suggested that there was significant difference between the groups (P 0.05). Conclusion Anti- Foxp3dsFv-IL-6 fusion protein can inhibit the differentiation of colon cancer cells by interfering with the imbalance of Treg/Th17. Key words: Anti-Foxp3dsFv-IL-6 fusion protein; Treg; Th17; Colon cancer cell line
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