Abstract
Objective To evaluate whether the protein SP70 could be used as a serum biomarker for the diagnosis of non-small cell lung cancer (NSCLC). Methods Polyclonal antibody was prepared by immunizing New Zealand rabbit with SPC-A1 cells.Sandwich ELISA was carried out by using newly-prepared polyclonal antibody(PcMb)coating assay plates,monoclonal antibody(McAb)NJ001 and HRP goat anti-mouse antibody as primary antibody and labeling antibody respectively.After optimizing the experiment conditions,serum from 175 lung cancer patients [80 NSCLC adenocarcinoma,70 NSCLC squamous carcinoma and 25 small cell lung cancer(SCLC)],25 benign lung disease(BLD)patients and 300 healthy controls (HC) were examined.CEA,NSE,CYFRA21-1 were measured by ECLIA for comparison. Results Positive rates of NSCLC adenocarcinoma,NSCLC squamous carcinoma,SCLC and BLD were 68.8%,51.4%,16.0% and 12.0% respectively,obviously higher than that of HC(7.3%).NSCLC(adenocarcinoma,Squamous carcinoma) had significantly higher positive rate than SCLC(60.7% vs 16.0%,χ2=17.23,P<0.05)and BLD(60.7% vs 12.0%,χ2=20.41,P<0.05).Among 68 NSCLC patients who had definite staging,positive rates at early stage(Ⅰ/Ⅱ,n=30) reached up to 76.7%.Meanwhile,positive rates of CEA,NSE and CYFRA21-1(32.7%,18.0% and 37.3%)were significantly lower than the targeting antigen to McAb NJ001 in NSCLC(60.7% vs 32.7%,χ2=23.63,P<0.05;60.7% vs 18.0%,χ2=57.22,P<0.05;60.7% vs 37.3%,χ2=16.34,P<0.05). Conclusions It showed high positive rates of SP70 in the serum of NSCLC patients,which suggested that SP70 might be a potential valuable biomarker for the diagnosis of NSCLC.(Chin J Lab Med,2012,35:554-558) Key words: Lung neoplasms; Carcinoma; non-small-cell lung; HSP70 heat-shock proteins; Tumor markers,biological; Enzyme-linked immunosorbent assay
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