A Preliminary Study of The Short Circuit Current ( Isc) Responses of Endometria from Estrum and Bilateral Ovariectomy Female Rats

Abstract

Objective To investigate the property of the endometrium ion transport in the normal estruation and ovariectomize rats. Methods The basic electrophysiological property of the endometrium was carried out by mean of the short circuit current (I sc ) recording with the rats. Results The baseline I sc and the transepithelial resistance (R te ) were different between the estruation and ovariectomized groups. Apical application of Na + channel blocker, amiloride (10 μmol/L), or CFTR Cl − channel agonist, forskolin (10 μmol/L) did not significantly affect the I sc in the two groups. However, the I sc in the model group was more sensitive to the CFTR Cl − channel blocker (glibenclamide, 1 mmol/L, apical) decreased about 27.0% (P − channel blocker, DIDS (4,4′-diisothiocyanostilbene-2,2′-disulfonic, 100 μmol/L), the I sc was much more sensitive, percentage almost to 36.52% (P + −K + −2Cl − cotransport, didn't significantly reduce the I sc in the two groups. Conclusion The baseline I sc and membrane resistance in the ovariectomize group is much higher than that in the normal estruation group, and the endometrial epithelium Cl − secretion may be mediated by predominantly calcium-dependent Cl − channels (CACC) and activating adenylate cyclase and apical cAMP-dependent Cl − channels (CFTR) in the model group with minor contributions in the control. Furthermore, the endometrial epithelium responses to different stimulants exists difference in the control and model group a likely mechanism for the ovary hormone.