Abstract

The experiment studied the change of enzymes in mild and acute drop of temperature.The activities of CAT,SOD,POD and the content of MDA in Shuiyuan No.1 sea cucumber and cultured sea cucumber were analyzed.The results show that:(1)In terms of mild drop in temperature,the activity of CAT were trend to decreased and then increased in Shuiyuan No.1 sea cucumber and Dalian cultured sea cucumber,the activities of CAT were 402.78-424.32 U/mL and 409.81-430.08 U/mL in Shuiyuan No.1 sea cucumber and Dalian cultured sea cucumber;the activity of SOD and POD first increased and then decreased and then increased in Shuiyuan No.1 sea cucumber and Dalian cultured sea cucumber;the activities of SOD were 0.019-0.086 U/mL and 0.014-0.069 U/mL in Shuiyuan No.1 sea cucumber and Dalian cultured sea cucumber,the activities of POD were 0.000 45-0.000 89 U/mL and 0.000 24-0.000 66 U/mL in Shuiyuan No.1 sea cucumber and Dalian cultured sea cucumber;the activity of MDA first increased and then reduced in Shuiyuan No.1 sea cucumber and Dalian cultured sea cucumber,the content of MDA were 5.83-9.79 nmol/mL and 7.57-9.90 nmol/mL in Shuiyuan No.1 sea cucumber and Dalian cultured sea cucumber.(2)In terms of acute drop in temperature,the activity of CAT,SOD and MDA first increased and then reduced in Shuiyuan No.1 sea cucumber and Dalian cultured sea cucumber,the activities of CAT were 382.51-409.71 U/mL and 383.86-399.42 U/mL in Shuiyuan No.1 sea cucumber and Dalian cultured sea cucumber;the activities of SOD were 0.023-0.038 U/mL and 0.025-0.035 U/mL in Shuiyuan No.1 sea cucumber and Dalian cultured sea cucumber;the content of MDA were 5.83-9.79 nmol/ml and 5.57-9.90 nmol/mL in Shuiyuan No.1 sea cucumber and Dalian cultured sea cucumber;the activity of POD were first increased and then decreased and then increased again to reduce,the activities of POD were 0.000 20-0.000 70 U/mL and 0.000 18-0.000 40 U/mL in Shuiyuan No.1 sea cucumber and Dalian cultured sea cucumber.(3)Both the activities of CAT,SOD,POD and the content of MDA were changed in the different ways of dropping temperature and the changing in Shuiyuan No.1 sea cucumber was more sensitive than in Dalian cultured sea cucumber in the terms of mild and acute drop in temperature.The activities of CAT and POD was higher in Shuiyuan No.1 sea cucumber than in Dalian cultured sea cucumber;the content of MDA was larger in the terms of acute drop than in the terms of mild drop in temperature.These results revealed that there are different immune responses to sea cucumber in different drops of temperature patterns.Shuiyuan No.1 sea cucumber was more sensitive and has higher capacity of antioxidant than Dalian cultured sea cucumber in the low temperature environment.

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