Abstract
Adipose derived stem cells (ADSCs) have a great potential for tissue-engineering purposes, and they may be introduced in oral mucosa tissue engineering for urethroplasty. This study was aimed to develop a tissue-engineered oral mucosa through seeding oral keratinocytes (OKs) and adipose derived stem cells (ADSCs) on small intestine submucosa (SIS). From August 2018 to October 2019, an observational study was conducted in the laboratory of our hospital, to develop a tissue-engineered oral mucosa.SIS was obtained from porcine small intestine, and OKs and ADSCs were obtained from canine sources and were cultured and expanded in vitro. The two cell lines were seeded on the two surfaces of the SIS, and the cell-scaffold compound graft was cultured in an air fluid level for 1 week. The SIS exhibited a porous membrane, and no cells were found through HE staining. The model cultured with OK-SIS only formed a thin and loose epithelium. Whereas the model cultured with OK-SIS-ADSC was much thicker and denser. The co-cultured of ADSC and OK grew well on the SIS in which the OKs formed a multilayer of epithelium. So it is feasible to construct a tissue-engineered oral mucosa graft with ADSCs, OKs and SIS. The ADSCs contribute to a thicker epithelium formation.
Highlights
The treatment of complex urethra stricture or urethral defect remains one of the most difficult problems in clinical urology, and tissue engineering seems to be the best choice for urethroplasty
We found that the existence of adipose derived stem cells (ADSCs) could activate the proliferation and migration rate of oral keratinocytes (OKs)
Without the feeder layer of i3T3, nearly 2 weeks passed before the OKs covered 90% of the culture plate; these cells could only be subcultured for 2 generations before aging and had multiple shapes and sizes (Figure 1D)
Summary
The treatment of complex urethra stricture or urethral defect remains one of the most difficult problems in clinical urology, and tissue engineering seems to be the best choice for urethroplasty. Acellular matrices and cell-seeded constructs have been used in the clinical treatment of urethra stricture [1,2,3], and OK-seeded acellular matrices were demonstrated to work well in an animal urethroplasty study [4]. The introduction of ADSCs in tissue engineering may accelerate the angiogenesis process of the graft. We found that the existence of ADSCs could activate the proliferation and migration rate of oral keratinocytes (OKs). In the present study, we cultured OKs and ADSCs and seeded them onto acellular small intestine submucosa (SIS) to obtain a new type of tissue-engineered tissue for urethroplasty
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