Abstract
Selective removal of degradation inhibitors from lignocellulosic biorefinery with resins is a very effective and easy operation. However, the resin will be heavily contaminated after the detoxification. The regeneration and recycle of poisoned-resin are difficult but necessary to overcome commercial production cost. In this study, various methods were compared to regenerate the poisoned-resin and finally 5% H2O2 for 5 h and 1 M NaOH for 4 h were chosen. This combined technology can recover the adsorption efficiency of regenerated resin to 86.97 % and 80.49 % for bio-inhibitors. The Boyd model and the Van't Hoff equation were used to fit the kinetics and thermodynamics of the regeneration process, respectively. The poisoned-resin maintained more than 63.3 % adsorption efficiency to acetic acid after three regeneration cycles and adsorbed fewer sugars. Even using several cycles of regenerated resin to detoxify the crude acidic hydrolysate, the biocatalytic performance of xylose to xylonic acid was increased from 18.6 % to 44.6 %. Moreover, the alkali can be recycled during the regeneration process, which can avoid the use of large amounts of alkali. This work could provide a green and practical approach for poisoned-resin recycle, and especially promote the technological development of lignocellulosic hydrolysate fermentation.
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