Abstract
A new elution system is proposed for preparative electrophoresis with intermittent elution. A solution of acrylamide and crosslinking agent is allowed to polymerize in a Perspex cylinder. A dialysis membrane stretched on a flat fritted-glass disk is fixed at the bottom end. The elution chamber is created by pumping through an inlet a small volume of buffer between the membrane and the lower surface of the gel, the outlet being closed by a relay valve. The use of Perspex material instead of glass assures displacement of the gel in the cylinder as well as good water-tightness along the wall. At the time of elution, the valve is opened and the buffer is expelled from the chamber by hydrostatic pressure exerted on the upper surface of the gel. An electronic control unit regulates the time of operation of the different components. Comparison of the results obtained for the separation of bovine serum albumin polymers and protein mixtures solubilized in sodium dodecyl sulfate, with those obtained using the analytical system demonstrates the good resolution obtained by use of the present system.
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