Abstract
The disturbance of intestinal microorganisms and the exacerbation of type 2 diabetes (T2D) are mutually influenced. In this study, the effect of exopolysaccharides (EPS) from Lactobacillus plantarum JY039 on the adhesion of Lactobacillus paracasei JY062 was investigated, as well as their preventive efficacy against T2D. The results showed that the EPS isolated from L. plantarum JY039 effectively improved the adhesion rate of L. paracasei JY062 to Caco-2 cells (1.8 times) and promoted the proliferation of L. paracasei JY062. In the mice experiment, EPS, L. paracasei JY062 and their complex altered the structure of the intestinal microbiota, which elevated the proportion of Bifidobacterium, Faecalibaculum, while inversely decreasing the proportion of Firmicutes, Muribaculaceae, Lachnospiraceae and other bacteria involved in energy metabolism (p < 0.01; p < 0.05); enhanced the intestinal barrier function; promoted secretion of the gut hormone peptide YY (PYY) and glucagon-like peptide-1 (GLP-1); and reduced inflammation by balancing pro-inflammatory factors IL-6, TNF-α and anti-inflammatory factor IL-10 (p < 0.01; p < 0.05). These results illustrate that EPS and L. paracasei JY062 have the synbiotic potential to prevent and alleviate T2D.
Highlights
We subsequently examined the viable count of L. paracasei JY062 grown to the end of exponential in different carbon sources (Figure 1b)
Our study confirmed that the EPS extracted from L. plantarum JY039 have prebiotic potential to promote the proliferation of L. paracasei JY062, and verified that the EPS can enhance the adhesion of L. paracasei JY062 to Caco-2 cells in vitro
The preventive effects of L. paracasei JY062, EPS and EPS + L. paracasei JY062 on Type 2 diabetes (T2D) were explored in mice
Summary
Diabetes mellitus is a chronic inflammatory disease that exists widely in the world and seriously endangers health [1]. Organization and the American Diabetes Association, the incidence rate of adult diabetes in mainland China is as high as 12.8%. Diabetes has become an urgent public health problem in China and most developing countries [2]. An increasing number of clinical and animal studies have focused on the phenomenon of prevalent intestinal microorganism (IM) disturbance in patients with T2D. This is mainly evident from the differences in the composition and dominant flora of the IM and is related to the type of diabetes [4,5].
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