A Potential Polymeric Nanogel System for Effective Delivery of Chlorogenic Acid to Target Collagen-Induced Arthritis

Abstract

The severe lethal activity of bone tissue and cartilage as denote to rheumatoid arthritis (RA). Polyphenols are extensively used as a curative agent for many diseases due to its anti-inflammatory, antioxidant and immunomodulant properties in many inflammatory chronic conditions. The selective delivery of polyphenol to the affected joints side achieves a better treatment strategy. Nanogels are promising medication conveyance frameworks and are made out of cross-connected different polymeric networks. The present investigation focusing on the potential uses of folic acid (FA) conjugated polymeric nanogel system is made by the interconnection between chitosan (CS) and poly(2-ethyl-oxazoline) (CS-POX) used to encapsulate chlorogenic acid (CA) to deduce the inflammatory effects in collagen-induced arthritis (CIA). The different dose of CA (1, 5, and 10 mg/mL) encapsulated CS-POX nanogel assists to clinical evaluation of diverse biological parameters including arthritis index (AI), hind paw volume, histological analysis, vital organ weight percentage and quantitative measurement of cytokines including, interleukin (IL)-1β, IL-6, IL-17, tumor necrosis factor (TNF-α) and prostaglandin enzyme 2 (PGE2). The results proved the oral administrated polymeric nanogel effectively deduces the joint space narrowing, AI score, the hind paw and minimizes the weight of vital organs in CIA rats. The increased level of inflammatory-related immune components such as (IL)-1β, IL-6, IL-17, TNF-α, PGE2 and anti-CII antibody in blood serum and joint tissue samples were subsequently reduced in the CIA rats after the treated with FA conjugated CS-POX-CA nanogel.