Abstract
In the present study, an Actinomycetes strain KSA-2 was isolated from freshwater sediment samples of Kali River, Karwar, Karnataka, India. The strain KSA-2 was selected among seven isolates based on primary screening of antimicrobial activity against pathogenic organisms. The morphological physiological and biochemical characterizations were performed, the bioactive secondary metabolites were produced in liquid broth culture and was characterized by UV-Vis. spectroscopy and FTIR spectroscopy. Later, the potent KSA-2 strain was identified by 16S rRNA gene sequencing (1366 bp) and a phylogenetic tree was constructed and the strain KSA-2 was confirmed as Streptomyces thermocarboxydus strain KSA-2. Further, the characterization of methanolic extract by UV-Vis. and FTIR spectroscopy analysis revealed the presence of broad spectrum of antimicrobial and other compounds and alkyl halides, alkenes, sulfoxide, carboxylic acids, alkanes respectively.
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