A Potential Association Between Antibiotic Abuse and Existence of Related Resistance Genes in Different Aquatic Environments

Abstract

Bacterial antibiotic resistance has long been a public health concern worldwide. Although antibiotic abuse highly correlates with occurrence of resistant pathogens in hot spots like animal feedlots, it remains obscure how frequently these resistance genotypes would emerge and/or retain in natural circumstances. In this study, we monitored seven antibiotic resistance genes in various surface waters. All seven resistance genes were detectable in Puzih River samples, including strA (40.6 %), cmlA (29.7 %), blaTEM (9.1 %), tet(B) (8.5 %), sul1 (7.9 %), mecA (3.6 %), and tet(A) (2.4 %). Among these genes, strA was observed in four out of five sampling occasions during the 1.5-year monitoring period and most of the genes were detected at least two times over five samplings. These results imply that surface waters in Taiwan act as potential reservoirs for several resistance genotypes. Moreover, high prevalence of tet(A) (92.0 %) and sul1 (96.0 %) in swine farm wastewater samples suggests routine antibiotic usage and particularly, the fodder supplements could indeed be a risk factor to antibiotic resistance in environments. sul1, tet(A), blaTEM, and strA were detectable in domestic water treatment plants and reservoirs, suggesting that several resistance genotypes against antibiotics as streptomycin, ampicillin, tetracycline, and sulfonamides are likely to persist in natural circumstance and may consequently contaminate the drinking water systems.