Abstract
Objective: The present study was conducted to investigate the cytotoxicity and antimicrobial activity of zinc oxide nanoparticles (ZnO NPs) synthesized as eco-friendly technique from the leaf extract of Ipomoea pes-caprae (L.) R. Br. against human lung adenocarcinoma (A549), brain tumor (U87) cells, and human pathogens Salmonella typhi, Staphylococcus aureus, Klebsiella pneumonia, Pseudomonas aeruginosa, and Bacillus subtilis.
 Materials and Methods: The work was carried out with varying precursor (plant extract) volume to optimize the synthesis of ZnO NPs and it was confirmed by ultraviolet (UV)-visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) analysis, scanning electron microscopy, and atomic force microscope (AFM) characterization techniques and evaluate its cytotoxicity activity by 3-(4,5-dimethyl-2- thiazolyl)-2,5-diphenyl--tetrazolium bromide assay method, antimicrobial activity by disk diffusion method.
 Results: A peak at 320 nm with maximum intensity was observed at temperature of 80°C with pH of 8.0 in UV-visible spectroscopy confirmed the formation of ZnO NPs and we calculate the size of ZnO NPs from XRD data found as 15.8 nm. The FTIR analysis evaluated that the presence of different functional groups is carboxyl, amine, and phenolic compounds of leaves extract which are involved in the reduction of zinc ions and acts as capping the ZnO NPs. AFM microgram confirms that ZnO NPs were in nanorange and spherical in nature. The cytotoxicity activity of A549 and U87 cell lines treated with various concentrations of ZnO NPs showed a dose-dependent increase in cell inhibition and the half maximal inhibitory concentration value was calculated to be 7.8 μg/ml. The antibacterial activity of selected pathogens shows higher zone of inhibition.
 Conclusion: The present study reveals that synthesized ZnO NPs capping with various bioactive compounds present in the leaf of I. pes-caprae show promising activity of cancer cell lines and antimicrobial agents; hence, further detailed study may lead to develop at a novel phytomedicine for the anticancer and antimicrobial drugs.
Highlights
There is much focusing on natural products obtained from plants and herbal resources, Ipomoea pes-caprae consider as an example of phytocompounds richest resources
It has been observed that the ratio of plant extract to zinc acetate 20 ml/gm (i.e., 1 gm of zinc acetate in 20 ml of plant extract) was optimal concentration for the synthesis of Zinc oxide NPs (ZnO NPs) which gives yield 95%
Insufficient amount of bioactive compound present in 10 ml/gm of zinc acetate lowers the yield of ZnO NPs gives 70%, whereas 30 ml/gm of zinc acetate yield 95% nearly same amounts of ZnO NPs that yield in 20 ml/gm (Fig. 1)
Summary
There is much focusing on natural products obtained from plants and herbal resources, Ipomoea pes-caprae consider as an example of phytocompounds richest resources. I. pes-caprae is an ancient plant employed in the treatment of headache and various types of inflammation including jellyfish sting dermatitis [2]. Biosynthesis of ZnO NPs was reported using plant extracts including Aloe vera (leaf) [15], Nephelium lappaceum L. Some reports show biosynthesized silver NPs from I. pes-caprae leaf extracts showed remarkable anticancer activity against liver cancer cells (A549) and human brain tumor cancer cell line (U87) and effective antibacterial activity [24]
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Asian Journal of Pharmaceutical and Clinical Research
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
