Abstract
A possible role of aliphatic alcohols and acetone in ribosome-catalyzed esterification, “fragment reaction”, and binding of aminoacyl ribooligonucleotides to ribosomes is suggested. It is proposed that formation of hydrogen-bonded complexes of 3′ or 2′ hydroxy groups of tRNA, CCA-oligonucleotide, N-acylaminoacyl or aminoacyl ribooligonucleotides with aliphatic alcohols or acetone hydrate provides an additional nucleophilic binding center (alcohol hydroxy group) which helps to anchor the substrate on ribosomes. Such a binding center may partially substitute for those originally present in an intact tRNA molecule. The complex of tRNA with an aliphatic alcohol resembles aminoacyl tRNA, which may account for a tRNA-dependent ribosome-catalyzed esterification. To explain differences in reactivity of straight- and branched-chain alcohols two possible mechanisms are invoked: esterification and solvolysis of hydrogen-bonded complexes of alcohols with tRNA.
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