Abstract
Abstract A double-stranded RNA of a size expected for the replicative form of BMV RNA 4, the smallest of the four RNAs of brome mosaic virus, was isolated from infected leaves by a procedure involving removal of single-stranded RNA by precipitation in 2 M LiCl, followed by cellulose chromatography and polyacrylamide gel electrophoresis. Like the other double-stranded RNAs of BMV, it had characteristics of a replicative form, such as distinctive elution patterns from cellulose and hydroxyapatite columns, and purple staining with toluidine blue O. It coelectrophoresed in 2.5% polyacrylamide gels with double-stranded RNA synthesized in vitro by Qβ replicase with BMV RNA 4 as template. The origin and mode of replication of BMV RNA 4 is discussed.
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