Abstract
Abstract This work aimed to explore a pheromone effect during food handling using food choice field experiments with Messor arenarius ants. The results showed that the proportion of times ants touched the two food items placed in the artificial food patch (collection point) with their antennae was significantly higher in their first collection attempt than in subsequent attempts. However, when the soil was replaced after each collection attempt, there were no significant differences in the proportions of cases in which ants touched the two food items with their antennae between the first and subsequent food collection attempts. This difference may indicate that ants deposited a pheromone in their first food collection attempt that faded away when the soil in the patch was replaced after each collection attempt.
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