Abstract

The formation of an insoluble complex between Mg ++, sodium fluoride and ATP was studied. The precipitation of ATP was dependent on the time of incubation and on the concentration of ATP, Mg ++ and sodium fluoride. The requirement for sodium fluoride was not replaced by sodium or potassium chloride but the requirement for Mg ++ was replaced by La +++ and Sn ++. Precipitation was stimulated by cAMP but was inhibited at high ionic strength and by EDTA, dATP, ADP and sodium pyrophosphate. The complex was slightly soluble in acid and completely soluble in alkali. Formation of this complex could result in errors in in vitro assays for enzymic phosphorylation of proteins and nucleic acids by acid precipitation.

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