Abstract
Hedysarum zundukii Peschkova is one of the Fabaceae endemics of the flora of the Lake Baikal west shore. Because of its very poor renewal by seed production and seedling appearance biotechnological method, clonal micropropagation has been elaborated in order to improve its chances of conservation. A protocol for clonal micropropagation, including introduction, propagation, rooting, acclimatization, field cultivation and prolonged cold storage has been elaborated. Half-dose MS salts, benzylaminopurine 1 mg/dm3 and 2% sucrose were optimal components of the medium for clonal micropropagation. Sucrose was the superior carbon source by comparison with glucose and maltose. It was found that some agar brands were better for propagation, whereas other ones were better for rooting. Transplants produced from acclimatized plantlets vegetated successfully in field conditions, but did not survived in Irkutsk after wintering. However, the same transplants planted into their natural population survived successfully. Micropropagated plantlets retained their ability for propagation in vitro after 10 -12 months of cold storage at 4℃ with illumination. It was concluded that clonal micropropagation may be used as an additional means for conservation of H. zundukii.
Highlights
Hedysarum zundukii Peschkova (HZ) is one of many endemics of Fabaceae Lindl. family of the flora of the Lake Baikal west shore [1,2]
The aim of this work was to elaborate clonal micropropagation (CMP) of HZ in order to include it into a system of measures directed towards conservation of this plant species
HZ bean segments were gathered in 2008 and 2009 mainly at the Cape Zunduk and the river of the same name on the west shore of Lake Baikal in Pribaikal National Park. 19 intact mature seeds of the mean weight were scarified on thin file and exposed for 1 minute in 70% ethanol for 15 minutes in 1% solution of NaOCl with added Twin-20 and washed three times for 5 min. each in sterilized water
Summary
Hedysarum zundukii Peschkova (HZ) is one of many endemics of Fabaceae Lindl. family of the flora of the Lake Baikal west shore [1,2]. Family of the flora of the Lake Baikal west shore [1,2] It is listed in the Irkutsk Regional Red Book [3] and Russian Federal Red Book [4]. Containers with plants were cultivated for the first 10 days in a growing room at 23 ̊C and illuminated with fluorescent lamps 16 h per day. They were covered with transparent plastic film in order to decrease excessive transpiration. After that, they were transferred to a greenhouse with natural illumination and unregulated temperature.
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