Abstract
IN previous papers, Nakao et al.1,2 reported that adenosine triphosphate (ATP) is regenerated in erythrocytes stored for more than several weeks or treated with fluoride3, when the cells are incubated with nucleoside and adenine at 37° C. In these aged or fluoride-treated cells, most of the nucleotides and other acid-soluble phosphate esters were decomposed almost completely and energy metabolism almost entirely stopped. To elucidate the mechanism of adenosine triphosphate synthesis in such erythrocytes, the following observations were carried out :
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